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Registros recuperados: 102
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Interactions between Crassostrea virginica larvae and Deepwater Horizon oil: Toxic effects via dietary exposure ArchiMer
Vignier, J.; Rolton, A.; Soudant, Philippe; Chu, F. L. E.; Robert, Rene; Volety, A. K..
The Deepwater Horizon (DWH) disaster released crude oil in the Gulf of Mexico for 87 days, overlapping with the reproductive season and recruitment of the oyster Crassostrea virginica. The pelagic larval life stages of C. virginica are particularly vulnerable to contaminants such as polycyclic aromatic hydrocarbons (PAHs) and oil droplets. Based on their lipophilic properties, PAHs and oil droplets can adsorb onto phytoplankton and filter-feeding C. virginica larvae may be exposed to these contaminants bound to suspended sediment, adsorbed onto algal and other particles, or in solution. This study examined the effects of exposure of C. virginica larvae to algae mixed with DWH oil. In a 14-day laboratory exposure, 5 day-old C. virginica larvae were exposed...
Tipo: Text Palavras-chave: Deepwater Horizon oil; Droplet; Oyster larvae; Dietary exposure; PAH.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00473/58423/61445.pdf
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Interet du compteur de particules ZB-ZBI et de l'analyseur C1000 pour la numeration des algues unicellulaires de culture. ArchiMer
Robert, Rene.
Marine unicellular algae growth rates were studied with: a Coulter Counter in conjunction with a channelizer, and a hemacytometer. Compared to the hemacytometer a good precision of the culture density was observed when the Coulter Counter was used in conjunction with a channelizer. Nevertheless care had to be taken when determining the growth of the diatoms.
Tipo: Text Palavras-chave: Laboratory equipment; Growth; Particle counters; Laboratory culture; Algal culture.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/publication-1777.pdf
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Investigation of marine bivalve morphology by in vivo MR imaging: first anatomical results of a promising technique ArchiMer
Pouvreau, Stephane; Rambeau, Marc; Cochard, Jean-claude; Robert, Rene.
Classically, investigation of the internal morphology and composition of molluscs and especially bivalves relies on destructive method (biometry, biochemistry and histology). These techniques have given essential information, but in contrast are time consuming and lead to the irreversible loss of the animal while they don't allow integration of the various levels of molecular-to-organism functioning. The aim of this study is to analyze for the first time the potential of NMR (nuclear magnetic resonance) imaging (MRI) to depict, with sufficient resolution and satisfactory contrast, the anatomy of a bivalve model, the Pacific oyster, Crassostrea gigas, without opening it. MRI experiments were carried out at 19 °C in several non-anaesthetized adult Pacific...
Tipo: Text Palavras-chave: In vivo physiology; Aquaculture; Anatomy; MRI; Pacific oyster.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1819.pdf
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La production aquacole de coquille Saint-Jacques (Pecten maximus) et le soutien scientifique à l'operation pilote ArchiMer
Dao, Jean-claude; Barret, Jean; Carval, Jean-pierre; Connan, Jean-paul; Corre, Christian; Devauchelle, Nicole; Fleury, Pierre-gildas; Laurent, Pascale; Mazuret, Michel; Miner, Philippe; Mingant, Christian; Muzellec, Marie-louise; Robert, Rene.
Aquaculture production of scallop has been identified by a series of technical operations during the past ten years programme under a partnership involving the fishermen organizations with regional and national grants. 3 million juveniles have been produced annually, giving a potential recapture by fishing of 100 to 120 tonnes 3 years later. Results in terms of technical and economical approach have pointed out the principal unknown factors responsable for high costs. 7he pilot programme aims to increase the production to reach a level of 5 million juveniles to support the fishery of the rade de Brest and induce new management of the resources. Two priorities were selected, control of the reproduction in the hatchery to obtain reliable larval and...
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Ano: 1995 URL: http://archimer.ifremer.fr/doc/00044/15494/12860.pdf
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Larval development and fatty acid composition of Ostrea edulis (L.) fed four different single diets from conditioning to pre-settlement ArchiMer
Gonzalez-araya, Ricardo; Robert, Rene.
Survival, growth and fatty acid composition of Ostrea edulis larvae (L.) fed four different single species, microalgal diets, Tisochrysis lutea (T), Chaetoceros neogracile (Cg), Skeletonema marinoi (Sm) or Tetraselmis suecica (Ts) from broodstock to pre-settlement, were studied. Lower larval growth (5.5m to 6.5m/d) was recorded in progeny continuously fed single S.marinoi or T.suecica, whereas good growth was achieved with single T.lutea (7.8m/d). Larvae, originated from broodstock receiving Sm or Ts, exhibited growth compensation when fed a bispecific balanced diet (TCg). This did not occur when broodstock and larvae were fed Cg or T, for which single or mixed diets led to similar larval growth. Furthermore, survival was high (>90%) regardless of...
Tipo: Text Palavras-chave: Broodstock conditioning; Diet; Larval development; Ostrea edulis.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00438/54986/56483.pdf
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Lethal and sub-lethal effects of Deepwater Horizon slick oil and dispersant on oyster (Crassostrea virginica) larvae ArchiMer
Vignier, J.; Soudant, P.; Chu, F. L. E.; Morris, J. M.; Carney, M. W.; Lay, C. R.; Krasnec, M. O.; Robert, Rene; Volety, A. K..
In April 2010, crude oil was spilled from the Deepwater Horizon (DWH) oil platform for 87 days, coincident with the spawning season and recruitment of the oyster, Crassostrea virginica, in the Gulf of Mexico. Impacts of acute exposures to surface-collected DWH oil (HEWAF), dispersed oil (CEWAF) and dispersant alone (Corexit 9500A®) on planktonic larval stages of C. virginica (veliger, umbo and pediveliger) were tested in the laboratory. Exposures to HEWAF, CEWAF and dispersant were toxic to larvae impairing growth, settlement success and ultimately survival. Larval growth and settlement were reduced at concentrations of tPAH50 ranging from 1.7 to 106 μg L−1 for HEWAF and 1.1–35 μg L−1 for CEWAF, concentrations well within the range of water sampled during...
Tipo: Text Palavras-chave: Deepwater Horizon oil spill; Crassostrea virginica; Corexit 9500A (R); Larvae; Growth; Settlement; PAH.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00345/45572/45181.pdf
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Manila clam Tapes philippinarum culture: Sediment-clam interactions ArchiMer
Goulletquer, Philippe; Robert, Rene; Trut, Gilles.
Manila clam (Tapes philippinarum) culture and sediment interactions were tested by comparing two rearing areas, including an oceanic ('Le-Ferret') and a more estuarine ('Les-Jacquets') sites in the bay of Arcachon (France). The growth of a calibrated clam population (10-mm spat) was monitored in these two areas with a concomitant sediment-water interface survey over a 1.5-year period. Two sites per area, including control and rearing plots, were sampled on a monthly basis. The potential clam farming impacts by bioturbation and interactions were examined at three sediment depths: 0-1, 1-2 and 2-10 cm. Moreover, the main hydrobiological parameters were measured on a weekly basis to establish a relationship between these parameters and sediment-water...
Tipo: Text Palavras-chave: Bay of Arcachon; Tapes philippinarum; Sediment water interface; Bioturbation; Clam culture; Bassin d'Arcachon; Tapes philippinarum; Interface eau sédiment; Bioturbation; Vénériculture.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-864.pdf
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Microalgues et nutrition larvaire en écloserie de mollusques ArchiMer
Robert, Rene; Trintignac, Pascal.
The production of live microalgae remains the only reliable technique for feeding bivalve mollusc larvae in a controlled environment. However, these algae must answer a number of size, nutritive value and mass-production criteria. The current knowledge in this field is assessed in this work which will lead us to the conclusion that only a few species meet these requirements.
Tipo: Text Palavras-chave: Algues fourrage; Mollusque; Ecloserie; Microalgue; Nutrition; Elevage larvaire.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/publication-6197.PDF
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Mise au point d'outils de gestion des gamètes mâles d'huîtres creuses Crassostrea gigas diploïdes et tétraploïdes à des fins de conservation et de diffusion ArchiMer
Brizard, Raphael; Boudry, Pierre; Haffray, Patrick; Labbe, C.; Suquet, Marc; Robert, Rene; Masse, G.; Pepin, Jean-francois; Saulnier, Denis.
In order to improve oyster culture, many research programs try and propose new methods aiming at improving genetically the product performances and quality (selection, polyploidisation). As for terrestrial animal production, the continuity of the different works as well as the spreading of the genetical progress would be made easier by the development of conservation techniques of the selected genotypes. Cryopreservation, which is often used in selection programs of terrestrial animals or fish farming species, is not always available for bivalves. This technique would enable the conservation of the gametes of individuals selected for their high growth potential, their resistance to diseases and/or their quality for a smaller cost and less risks than the...
Tipo: Text Palavras-chave: Tetraploïde; Diploïde; Concervation; Spermatozoides; Cryoconservation; Génétique; Crassostrea gigas; Huîtres.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3339.pdf
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New microalgae for the Pacific oyster Crassostrea gigas larvae ArchiMer
Ponis, Emanuele; Probert, Ian; Veron, Benoit; Mathieu, Michel; Robert, Rene.
The number of microalgae of high nutritional value actually available to mollusc hatcheries is limited to a few species and this represents a constraint for the industry. Fifteen microalgal species belonging to seven different taxonomic classes were selected from the Algobank-Caen microalgal culture collection of the University of Caen. An initial screening of these microalgae, based on their cytomorphological characteristics and growth performances, led to the selection of four species (Imantonia rotunda, Emiliania huxleyi, Pseudoisochrysis paradoxa and Diacronema vlkianum), which were then tested as food for Crassostrea gigas larvae. Two species (L rotunda and E huxleyi) were of poor food value for oyster larvae, while P paradoxa and D. vlkianum resulted...
Tipo: Text Palavras-chave: Biochemical composition; Larval nutrition; Mollusc hatchery; Microalgae.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1547.pdf
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Nouvelle méthode pour étudier la nutrition de jeunes larves de Crassostrea gigas (Thunberg) en milieu naturel. Premières données expérimentales ArchiMer
His, Edouard; Robert, Rene; Chretiennot-dinet, Marie-josèphe.
This paper describes a new technique for isolating unicellular algae which are thought to play a role ine the feeding fo bivalve larvae. The results are compared with the data obtained from experimental culture of bivalve larvae under controlled conditions. Effectively ingested algal strains can be isolated by this technique. The food value of one strain obtained as monsopecific culture is demonstrated.
Tipo: Text
Ano: 1985 URL: http://archimer.ifremer.fr/doc/00021/13255/10301.pdf
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Nursery function of coastal temperate benthic habitats: New insight from the bivalve recruitment perspective ArchiMer
Barbier, Pierrick; Meziane, Tarik; Foret, Martin; Tremblay, Rejean; Robert, Rene; Olivier, Frederic.
Marine habitat function has been typically investigated in terms of biogeochemical regulation but rarely in terms of population renewal, which is mainly controlled by recruitment dynamics. The recruitment phase is crucial for organisms with a bentho-pelagic life cycle, such as bivalves, and it regulates the population renewal success. This study provides new insight on the role of temperate benthic habitats on bivalve recruitment, as a function of nursery areas. Six dominant benthic habitats of the Chausey archipelago (Normandy, France) were studied. In each habitat, bivalve recruit assemblages were described at the end of two reproductive seasons. Furthermore, Ostrea edulis juveniles were immerged on each habitat during two months to compare growth...
Tipo: Text Palavras-chave: Benthic habitats; Bivalve recruitment; Nursery function; Ostrea edulis; Fatty acids.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00367/47863/47845.pdf
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Nutrional inadequacy of Nannochloris atomus and Stichoccocucs bacillaris for the oyster Crassostre gigas (Thunberg) larvae ArchiMer
Robert, Rene.
The evaluation of Nannochloris atomliS and StichoccocllS bacillaris as nutrient sources for oyster larvae has been attempted in the present work. After a study of their grawth and size distributions, these two species were used as food for Crassostrea gigas larvae. Despite praper sizes (2.5 to 20 µm3) the larval development of Crassostrea gigas was poor. Evaluation of ingestion and digestion of both algae, by means of epifluorescence microscopy, on 2 day and 6 day old larvae showed that they were normally ingested but weakly digested. Consequently, their nutritional inadequacy for larval molluscs may be also related to their indigestibility.
Tipo: Text Palavras-chave: Stichococcus bacillaris; Nannochloris atomus; Larvae; Nutrient sources; Huître; Crassostrea gigas; Nutrition; Stichococcus bacillaris; Nannochloris atomus; Larve; Algue fourrage; Huître; Crassostrea gigas; Nutrition.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-6198.PDF
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Nutritional value of six Pavlovophyceae for Crassostrea gigas and Pecten maximus larvae ArchiMer
Ponis, Emanuele; Probert, I; Veron, Benoit; Le Coz, Jean-rene; Mathieu, Michel; Robert, Rene.
Four unidentified new strains of Pavlovophyceae [Pavlova sp. AC 250 (M), Pavlova sp. AC 248 (Psh), Pavlova sp. AC 251 (Pth), Pavlova sp. AC 538 (Psm)] and two known species [Pavlova pinguis (Ppi), Rebecca salina (ex Pavlova salina, Rsa)] were characterized (productivity, size, dry weight, ash, gross composition, fatty acids, sterols) and their nutritional value in bispecific diets were evaluated both on Crassostrea gigas and Pecten maximus larvae. All microalgae exhibited poor food value for C gigas larvae. P. pinguis and R. salina did not sustain any growth, like the control starved larvae. No exo-toxicity of P. pinguis and R. salina was detected. These two species were weakly ingested by C. gigas larvae, while the four other pavlovophytes were...
Tipo: Text Palavras-chave: Biochemical composition; Larval nutrition; Nutritional value; Microalgae.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1558.pdf
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Observations complementaires sur les causes possibles des anomalies de la reproduction de Crassostrea gigas (Thunberg) dans le bassin d'Arcachon. ArchiMer
His, Edouard; Maurer, Daniele; Robert, Rene.
The spat production in the Bay of Arcachon has been recently disturbed. Bioassays using algae used as food for oyster larvae have shown that the organotin paints affect or inhibit growth of Isochrysis galbana and Chaetoceros calcitrans . Fresh waters coming into the bay and shore sediment did not affect the growth of these algae. Bioassays proposed by Woelke (1967) using embryos and larvae of Crassostrea gigas have shown that the quality of the sea water from Arcachon harbour has been improved a few months after the use of organotin paints has been prohibited. Likewise fresh waters are free from any micropollutants that could drastically disturb the reproduction of C. gigas in the bay.
Tipo: Text Palavras-chave: Marine mollusks; Pollution effects; Recruitment; Population number; Oyster culture.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/publication-1788.pdf
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Observations on larval development and settlement of Patinofecten yessoensis in hatcheries ArchiMer
Robert, Rene; Miner, Philippe; Mazuret, Michel; Connan, Jean-paul.
The influence of density on Patinopecten yessoensis larval development was studied during production of an F2 generation. Setting behaviour of P. yessoensis on PVC removable spat collectors, previously tested with Pecten maximus, was determined. Poor larval growth of P. yessoensis was observed at a larval density of 7 ·ml·1 and post larval settlement occurred mainly on the bottom of the baskets, as has been observed with the king scallop, P. maximus. The PVC collector is not suitable for either species of scallop.
Tipo: Text
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00090/20163/17816.pdf
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Ontogenic changes in the contents of dopamine, norepinephrine and serotonin in larvae and postlarvae of the bivalve Pecten maximus ArchiMer
Cann Moisan, Christine; Nicolas, Laurence; Robert, Rene.
In the present study, norepinephrine (NE), epinephrine (E), dopamine (DA) and 5-hydroxy-tryptamine (5HT) have been quantified by high performance liquid chromatography (HPLC) in Pecten maximus during larval and post-larval development. With average values ranging from 2 to 5 pg mug(-1) of protein, NE remained low through the whole larval life, while epinephrine (E) was undetected. DA and 5HT were the most abundant monoamines with significant variations between larval, pre-metamorphic and post-larval stages. During the first 20 days, corresponding to D larval and umboned larval stages, levels of DA and 5HT increased from 15 to 30 and 10 to 15 pg mug(-1) of protein, respectively. Then during the last week of larval life, at the approach of metamorphosis, DA...
Tipo: Text Palavras-chave: Pecten maximus; Metamorphosis; Post larvae; Larvae; Monoamines.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/2002/publication-568.pdf
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Optimisation de la production en écloserie de mollusques : Mise au point d'un pilote industriel de production phytoplanctonique en continu et des techniques associées pour leur intégration en écloserie commerciale ArchiMer
Angeri, S.; Bougaran, Gael; Connan, Jean-paul; Jouin, Jean-claude; Kaas, Raymond; Le Dean, Loic; Legrand, J.; Loubiere, K.; Lukomska, Ewa; Olivo, Erell; Picquet, Marie-lise; Pruvost, J.; Robert, Rene.
Les travaux présentés dans le cadre de ce rapport d'activité rendent compte des avancées réalisées sur le plan (1) biologique, avec d'une part une étude sur le comportement du genre Chaetoceros en culture continue et, d'autre part, les résultats d'expérimentations sur les performances d'élevages larvaires réalisés sur la base de cultures de microalgues en continu, et (2) technologique, avec la continuité des actions de développement du photobioréacteur destiné aux écloseries commerciales ainsi que des périphériques indispensables à son intégration dans le secteur aquacole.
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Ano: 2007 URL: http://archimer.ifremer.fr/doc/00304/41518/40726.pdf
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Optimizing conditions for the continuous culture of Isochrysis affinis galbana relevant to commercial hatcheries ArchiMer
Marchetti, Julie; Bougaran, Gael; Le Dean, Loic; Megrier, Cyril; Lukomska, Ewa; Kaas, Raymond; Olivo, Erell; Baron, Regis; Robert, Rene; Cadoret, Jean-paul.
Optimal culture conditions were investigated for the continuous culture of Isochrysis affinis galbana (T-Iso) by assessing the effects of irradiance, temperature, pH and nitrogen and phosphorus requirements. Assessment of the growth rate-irradiance relationship in a turbidostat experiment highlighted that an optimum irradiance efficiency for growth (E-mu) occurred at irradiance much lower than the saturating level. Furthermore, our modeling approach for productivity pointed out that an optimal set of irradiance and dilution rate could be achieved in order to maximize irradiance efficiency. Optimal conditions for growth were assessed for temperature, pH and irradiance by means of a factorial design experiment. The resulting model predicted that optimal...
Tipo: Text Palavras-chave: Isochrysis affinis galbana; Irradiance efficiency; Factor interaction; Nutrient limitation; Microalgae cultures.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00076/18736/16465.pdf
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Oyster sex determination is influenced by temperature - First clues in spat during first gonadic differentiation and gametogenesis ArchiMer
Santerre, Christelle; Sourdaine, Pascal; Marc, Nicolas; Mingant, Christian; Robert, Rene; Martinez, Anne-sophie.
The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMI, Cg-SoxE, Cg-beta-catenin, Cg-Foxl2/Cg-FoxI2os) and of Oyvlg, a germ cell marker, were investigated by real-time PCR in spat grown at different temperatures (18, 22, 25 and 28 degrees C). In parallel, gonadic differentiation, gametogenesis and sex ratios were assessed by histology at each of these temperatures. Whatever the temperature, Cg-DMI, Cg-SoxE, Cg-beta-catenin and Oyvlg expressions peaked at the same developmental stage, always after Cg-Foxl2/Cg-Foxl2os (around 40-44 dpf for spat grown at 18 degrees C). Temperatures...
Tipo: Text Palavras-chave: Oyster spat; Temperature; Sex ratio; TSD; Sex determination pathway.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00136/24767/22874.pdf
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